Protocols:

Drosophila embryo collection | PDF

Formaldahyde fixation of embryos | PDF

Antibody staining | PDF

Antibody staining with TSA amplificaiton | PDF

in vitro transcription (Make dig-labeled RNA probes) | PDF

in situ hybridization using Alkaline Phosphatase detection | PDF This protocol is based on Tomancak et al. (2002) with some modification

in situ hybridization using fluorescence detection | PDF                   A modification of the Alkaline Phosphatase protocol using fluoresence Tyramide Signal Amplification (TSA). Notes are included on in situ hybridization followed by antibody staining.