Home

Tools

• RNA-seq search
• Confocal search

Cell Types

• All cell types
• MP1
• H-cell
• H-cell sib
• iVUMs
• mVUMs
• MNB
• Midline glia
• Accessory cells

Development

• stages 5-6
• stages 6-9
• stages 10-11
• stages 11-13
• stages 13-17

Information

• help
• protocols
• references
• crews lab home
• contact us

Protocols:

Drosophila embryo collection | PDF

Formaldahyde fixation of embryos | PDF

Antibody staining | PDF

Antibody staining with TSA amplificaiton | PDF

in vitro transcription (Make dig-labeled RNA probes) | PDF

in situ hybridization using Alkaline Phosphatase detection | PDF This protocol is based on Tomancak et al. (2002) with some modification

in situ hybridization using fluorescence detection | PDF                   A modification of the Alkaline Phosphatase protocol using fluoresence Tyramide Signal Amplification (TSA). Notes are included on in situ hybridization followed by antibody staining.