Drosophila embryo collection | PDF
Formaldahyde fixation of embryos | PDF
Antibody staining | PDF
Antibody staining with TSA amplificaiton | PDF
in vitro transcription (Make dig-labeled RNA probes) | PDF
in situ hybridization using Alkaline Phosphatase detection | PDF This protocol is based on Tomancak et al. (2002) with some
in situ hybridization using fluorescence detection | PDF A modification of the
Alkaline Phosphatase protocol using fluoresence Tyramide Signal Amplification (TSA). Notes are included on in situ hybridization followed by antibody staining.